Filamentous fungi are ubiquitous organisms that play a significant role in industry, agricultural, drug development, and plant/human health. Fungal pathogens are the main factors charging of the most severe diseases affecting plants, leading to a reduction in yield and crop quality and causing enormous economic losses worldwide. Owing to the fundamental importance, Filamentous fungi have attracted more attention on molecular studies, especially for manipulation of the genome and the coding sequences. Creative Biogene is a leading company offering series of genetic service, including gene deletion/editing, RNAi, protein expression, and gene tagging procedures.
For more complex, multicellular organisms, the genetic manipulation of filamentous fungi is often more difficult due to the typically complex genetic basis of background. As a versatile genome editing technology, CRISPR/Cas9 system is revolutionizing technology that shows great promise in research and application of filamentous fungi. Partial characterization of the functions of specific genes important to the biology of fungal species can be achieved by either knockout or knockin experiments followed by phenotypic analysis of the mutant strains.
Our CRISPR-Cas9 mediated genetic engineering system is designed for simple, marker-free, effective and versatile use in a broad spectrum of fungi. We have three editing routes to understand better the function of genes and their regulation.
Compared with prokaryotic, filamentous fungal are much more complex, frequently facing problems such as inefficient transformation. There are a number of different transformation systems that have been developed in a broad spectrum of filamentous fungi.
